Fig. 2: 3D-LAFM density map of annexin-V.

a, b 2D slices of the 3D-LAFM detection stack (a) (see Fig. 1b, right, Supplementary Movie 1) and the 3D-LAFM density map (b) (Supplementary Movie 2). Selected i-j planes (blue) and j-k planes (red) are displayed. Yellow dots: Positions of the three example local maxima with different h-values (low, medium, and high) from the first particle in the raw AFM frame (see Fig. 1b, yellow dots). Left: Schematics of the i-j and j-k plane 2D slices. c Cartoon of A5-monomer crystal structure (PDB 1AVR) from the liquid-facing side (top) and in a side view (bottom). d Surface presentation of A5-trimer from the liquid-facing side. Dashed line: A5-monomer. e 3D-LAFM density map in Chimera, viewed in top-view (i-j plane) projection. Grayscale: probability density. f High-density surface (top-view) presentation of the 3D-LAFM density map. False-color scale represents height values, similar to the surface presentation of the structure in (d). g Comparison of the A5-monomer liquid-facing surface in the crystal structure (left), 3D-LAFM density map (middle), and the 3D-LAFM high-density surface (right). h Fourier Shell Correlation (FSC) analysis of masked 3D-LAFM half-density maps. Arrowheads in c–f: Annexin-repeats I, II, III, and IV.