Fig. 4: Single-cell sequencing confirmed the subtype-specific splicing landscape.
From: Splicing diversity enhances the molecular classification of pituitary neuroendocrine tumors
a Sankey diagram showing the changes in differential splicing events from bulk to scRNA-seq data. Heatmap showing the 117 splicing characterizations in the pseudo-bulk from scRNA-seq. b Read coverage showing the inclusion of splicing events on single-cell clusters. c Sankey diagram showing the changes in differentially expressed RBPs from bulk to scRNA-seq data. Heatmap showing the 108 dysregulated RBPs. d Ternary plot of the relative percentage of splicing events based on their presentations in PIT1 patients. The PSI of each splicing event is normalized across all subgroups. Each splicing event (node) has three coordinates indicating that a% is the normalized PSI detected in GH clusters, b% is the normalized PSI detected in PRL clusters, and c% is the normalized PSI detected in TSH clusters. And a + b + c = 100. e UMAP plots showing cells from the PIT1 lineage. f, g UMAP plots showing cells from the PIT1 lineage with detected reads for the NCAM1 event (f) and the NFE2L1 event (g). h Ternary plot of the relative percentage of splicing events based on their presentations in TPIT and SF1 patients. i UMAP plots showing cells from the TPIT lineage. j, k UMAP plots showing cells from the TPIT lineage with detected reads for the ENAH event (j) and the ARFGAP1 event (k). l Venn diagram showing the overlap between differential splicing events verified by pseudo-bulk and single-cell level. P-values are calculated by a two-sided Wilcoxon rank-sum test. m Differentially spliced events verified by pseudo-bulk in subtypes are ranked by the P-values. P-values are calculated by a two-sided Wilcoxon rank-sum test. n Box plot showing the APP splicing events in the cells of all tumor clusters (n = 46,051). Different colors represent different subtypes. The boxes indicate median (center), Q25, and Q75 (bounds of box), the smallest value within 1.5 times interquartile range below Q25 and the largest value within 1.5 times interquartile range above Q75 (whiskers). P-values are calculated by a two-sided Wilcoxon rank-sum test. Source data are provided as a Source Data file.
