Fig. 1: Actin stress fibers are remodeled during senescence to support cell enlargement and the SASP.
From: Cell enlargement modulated by GATA4 and YAP instructs the senescence-associated secretory phenotype
a Abundance of the indicated mRNAs for the indicated day after senescence induction by 12 Gy IR in IMR90 cells expressing hTERT (IMR90-hTERT cells) analyzed by RT-qPCR (left, n = 3 biologically independent samples, mean ± SEM, one-way ANOVA test with Tukey’s multiple comparisons test). Cell size for the indicated day analyzed by Fluorescence-Activated Cell Sorting (right, n = 4 biologically independent samples, mean ± SEM, one-way ANOVA test with Tukey’s multiple comparisons test). b Representative images of actin stress fiber staining (left, n = 3 biologically independent experiments). Scale bar, 50 μm. Phalloidin intensity, cell size, and phalloidin intensity normalized to cell size analyzed by ImageJ software (right, n = 57, 36, 46, and 47 randomly chosen cells from 3 biologically independent experiments for PRO, SEN, SEN+LatA, and SEN + Y27632, respectively, box plots show the 1st and 3rd quartiles; whiskers show the minimum and maximum values, one-way ANOVA test with Tukey’s multiple comparisons test, AFU denotes arbitrary fluorescence units). c Quantification of the percentage of SA-β-gal positive cells (n = 9 biologically independent samples, mean ± SEM, one-way ANOVA test with Tukey’s multiple comparisons test). d Abundance of the indicated proteins analyzed by immunoblotting (left, n = 3 biologically independent experiments) and abundance of the indicated mRNAs analyzed by RT-qPCR (right, n = 3 biologically independent samples, mean ± SEM, one-way ANOVA test with Tukey’s multiple comparisons test). e Abundance of the indicated proteins analyzed by immunoblotting (left, n = 3 biologically independent experiments) and abundance of the indicated mRNAs analyzed by RT-qPCR (right, n = 3 biologically independent samples, mean ± SEM, one-way ANOVA test with Tukey’s multiple comparisons test). PRO and SEN denote proliferating and senescent IMR90-hTERT cells, respectively. LatA denotes Latrunculin A. Source data are provided as a Source Data file.
