Fig. 7: Expression of RHOU associates with the SASP and senescence in vivo.

a Adipose tissues were taken from young (4.5-month-old) and old (26-month-old) male C57BL/6 mice and abundance of the indicated proteins was analyzed by immunoblotting (left, n = 3 biologically independent samples). Adipose tissues were taken from normal chow-fed and high-fat diet (HFD)-fed male C57BL/6 mice and abundance of the indicated proteins was analyzed by immunoblotting (right, n = 4 biologically independent samples). b Box plot analysis comparing the abundance of the indicated genes between RHOU^low and RHOU^high adipose tissue samples in human aging. Samples from adipose tissues (subcutaneous) in the Genotype-Tissue Expression (GTEx) database were divided into two age groups [‘Young’ (20–39 years old) and ‘Old’ (60–79 years old)], and subsequently categorized into 2 groups [RHOU^low (bottom 10%) and RHOU^high (top 10%)] according to RHOU expression (n = 10, 11, 23, and 24 biologically independent individuals for Young RHOU^low, Young RHOU^high, Old RHOU^low, and Old RHOU^high, respectively, box plots show the 1st and 3rd quartiles; whiskers show the minimum and maximum values, one-way ANOVA test with Tukey’s multiple comparisons test, TPM denotes transcripts per million). c Model of how cell enlargement antagonistically regulated by GATA4 and YAP supports the SASP program¹⁴². Source data are provided as a Source Data file.