Fig. 4: Liver-specific Axin1 knockout attenuates HIRI-induced hepatocyte injury and inflammation in mice. | Nature Communications

Fig. 4: Liver-specific Axin1 knockout attenuates HIRI-induced hepatocyte injury and inflammation in mice.

From: Axin formation inhibitor 1 aggravates hepatic ischemia‒reperfusion injury by promoting the ubiquitination and degradation of PPARβ

Fig. 4

A Construction of Liver -specific Axin1 knockout mice. B The efficiency of Axin1 knockdown was measured by Western blot (n = 6). C, D H& E staining was used to detect liver tissue injury in mice (n = 6, P = 0.0001). E, F ALT and AST expression level in serum of mice in each group (n = 6, P = 0.0006, <0.0001). G LDH expression level in serum of mice in each group (n = 6, P = 0.0352). H–K The expression of apoptosis-related proteins was detected by Western blot (n = 6, P = 0.000888, 0.020666, 0.004120). L, M Apoptotic cells were detected by Tunel staining (n = 6, P = 0.0001). N, O CD11b positive cells were detected by immunofluorescence staining (n = 6, P = 0.0003). P, Q Ly6G positive cells were detected by immunofluorescence staining (n = 6, P = 0.0005). R The expression of inflammatory factors was detected by qRT-PCR (n = 6, P = 0.001686, 0.000398, 0.009210). HKO hepatocyte knockdown, IR ischemia reperfusion, LDH lactate dehydrogenase, ALT alaninea minotransferase, AST aspartate aminotransferase. Two-tailed t-test. The data are presented as mean ± SD. Three biologically independent experiments. Source data are provided as a Source Data file.

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