Fig. 5: TaTCP6 interacts with TaPHR2.
From: TaTCP6 is required for efficient and balanced utilization of nitrate and phosphorus in wheat
a Heatmaps showing the relative expression changes (z-normalized) of genes mediating phosphorus uptake and utilization determined by RNA-seq in TaTCP6-OE. The values used to generate the heatmaps are the average FPKMs of two independent RNA-seq replicates. b Pi contents of Mock and TaTCP6-OE wheat plants. wheat seedlings grown for 2 weeks were sampled for analysis Pi contents. Data are mean ± SD (n = 6); P values are from two-sided Student’s t-tests. Exact P values and source data are provided as a Source Data file. c Yeast two-hybrid (Y2H) analysis of protein interaction between TaKAN1, TaMYR1, TaCLK1, TaNIGT1, and TaTCP6. TaTCP6TCP only contains the TCP domain (Pfam: PF03634). d A schematic diagram of the protein domain structure of several GARP family transcription factors. e–i Yeast two-hybrid analysis of protein interaction domain between TaRLI1, TaPHR2, and TaTCP6. Interaction between TaRLI1, TaPHR2, and TaTCP6 confirmed by Y2H (e), bimolecular fluorescence complementation (BiFC; f), firefly luciferase complementation imaging (LCI; g), pull-down (h), and co-immunoprecipitation (Co-IP; i) assays. Scale bars, 10 μm. These experiments in (h, i) were repeated three times independently with similar results.
