Fig. 3: IL-1β neutralizing antibody alleviates the aggravated obesity by AMPK deficiency in myeloid cells.
From: Macrophage SUCLA2 coupled glutaminolysis manipulates obesity through AMPK
a Schematic diagram of mice feeding and this image was created in BioRender. Zang, Y. (2025) https://BioRender.com/e16k510. b–n Male AMPKαfl/fl mice and LysM-Cre, AMPKαfl/fl mice with C57BL/6 background at the age of 6 weeks were fed HFD with or without IL-1β neutralizing antibody (1 mg/kg, twice one week) to explore the obesity development. Immunofluorescent staining of IL-1β in BAT and ScWAT (b), body weight gain (c, n = 5 mice), relative fat and lean mass (d, n = 5 mice), the weight of Liver (e, n = 5 mice), BAT (f, n = 5 mice), and ScWAT (g, n = 5 mice), representative H&E staining of the liver, BAT and ScWAT (h), insulin tolerance test (i, n = 5 mice), the rectal temperature in cold exposure at 4 °C for different times (j, k, n = 5 mice), immunohistochemical staining of UCP-1 in BAT (l), the proinflammatory genes of ScWAT (m, Il1b, Tnfa, Nos2, Ccl2 and F4/80: n = 5 mice in each group, Il6: n = 4 mice in LysM-Cre, AMPKαfl/fl + IL-1β mAb group and n = 5 mice in other group), and the immunohistochemical staining of F4/80 in BAT and ScWAT (n). Data are presented as the mean ± SEM, groups were compared by two-way ANOVA followed by Fisher’s LSD test (c–g, i–k, m). P < 0.05 was considered to be statistically significant.
