Fig. 4: Decidualization defects in Grb2d/d mice and hESCs. | Nature Communications

Fig. 4: Decidualization defects in Grb2d/d mice and hESCs.

From: GRB2 regulation of essential signaling pathways in the endometrium is critical for implantation and decidualization

Fig. 4

a A decrease of the stimulated/control uterine weight ratio in Grb2f/f and Grb2d/d mice at decidualization day 5 (n = 5 for Grb2f/f and n = 4 for Grb2d/d mice). The results represent the mean ± SEM. ***p = 0.0001 by two-tailed unpaired t-test. b Hematoxylin and eosin (H&E) staining in control and stimulated horn of Grb2f/f and Grb2d/d mice at decidualization day 5. c RT-qPCR analysis for the expression of decidualization marker genes, Bmp2, Fst, Fkbp5, and Wnt4, in the uteri of Grb2f/f and Grb2d/d mice (n = 4 per genotype). The results represent the mean ± SEM. ***p < 0.0001, ***p < 0.0001, ***p < 0.0001, ***p < 0.0001, *p = 0.0435, **p = 0.0045, **p = 0.0034, **p = 0.0042 by Ordinary one-way ANOVA test. d The expression of GRB2 in hESCs from control women without (Ctrl) and with (Eosis) endometriosis (n = 22 per group). The expression of GRB2, IGFBP1, and PRL levels in hESCs and after silencing with siRNA in decidualized HESCs. Each treatment was performed with at three biological replicates (n = 3 per group and time point). The results represent the mean ± SEM. ***p = 0.0001, **p = 0.0085, *p = 0.0200, **p = 0.0035, ***p = 0.0004, and ***p < 0.0001, by Ordinary one-way ANOVA test. Source data are provided in the Source Data file.

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