Fig. 8: A decrease of FOXA2 expression in the uteri in Grb2^d/d mice at GD 3.5.

a The number of grands in the uteri of Grb2^f/f and Grb2^d/d mice at GD 3.5 (n = 6). The results represent the mean ± SEM. ***p < 0.0001 by two-tailed unpaired t-test. b Immunohistochemistry analysis of FOXA2 in the uteri of Grb2^f/f and Grb2^d/d mice at GD 3.5 (n = 5 per treatment). The results represent the mean ± SEM. ***p < 0.0001 by two-tailed unpaired t-test. c Venn diagram of identifying genes that overlapped in RNA-Seq data between Foxa2^d/d and Grb2^d/d mice at GD 3.5. d A heatmap of differentially expressed genes in uterus of control, Foxa2^d/d and Grb2^d/d mice at GD 3.5 from RNA-Seq data (n = 4 per genotype). e Heatmap of Grb2 and Foxa2-related genes in the uteri of Foxa2^d/d and Grb2^d/d mice at GD 3.5 (n = 4 per genotype). Source data are provided in the Source Data file.