Fig. 10: The immune and lung metastasis inhibition effects of different treatments in RM-1 tumor-bearing C57BL/6J male mice. | Nature Communications

Fig. 10: The immune and lung metastasis inhibition effects of different treatments in RM-1 tumor-bearing C57BL/6J male mice.

From: Engineering nanozyme immunomodulator with magnetic targeting effect for cascade-enzyodynamic and ultrasound-reinforced metallo-immunotherapy in prostate carcinoma

Fig. 10

Flow cytometry analysis of matured DCs (a), CD8+ T cells (b), and MDSCs (c) in tumors after various treatments (G1: Control, G2: US, G3: ZFP, G4: ZFPG, G5: ZFP + US, G6: ZFPG + US, n = 3 biologically independent samples, mean ± SD, statistical significance was calculated by one-way ANOVA test); matured DCs (d), CD8+ T cells (e), and MDSCs (f) in lymph nodes after various treatments (G1: Control, G2: US, G3: ZFP, G4: ZFPG, G5: ZFP + US, G6: ZFPG + US, n = 3 biologically independent samples, mean ± SD, statistical significance was calculated by one-way ANOVA test); matured DCs (g), CD8+ T cells (h), and MDSCs (i) in the spleen after various treatments (G1: Control, G2: US, G3: ZFP, G4: ZFPG, G5: ZFP + US, G6: ZFPG + US, n = 3 biologically independent samples, mean ± SD, statistical significance was calculated by one-way ANOVA test); the levels of IFN-γ (j) and TNF-α (k) in the tumor after different treatments (G1: Control, G2: US, G3: ZFP, G4: ZFPG, G5: ZFP + US, G6: ZFPG + US, n = 3 biologically independent samples, mean ± SD, statistical significance was calculated by one-way ANOVA test); l schedule of the prostate cancer lung metastasis experiment; m fluorescence images of metastatic lesions in Luc-RM-1 tumor-bearing black mice after different treatments; n representative images of the Bouin’s Fixative-stained whole lungs (white arrows point to the lesions); and o representative H&E-stained lung tissue sections (G1: Control, G2: US, G3: ZFP, G4: ZFPG, G5: ZFP + US, G6: ZFPG + US). Source data are provided as a Source Data file.

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