Fig. 2: RNF167 regulates RNA virus-induced innate immune response.

a qPCR and immunoblot analysis of RNF167 expression levels in wild type (WT) and RNF167-knockout HEK293 cells (n = 3). b qPCR analysis of mRNA expression levels of IFNB, the related ISGs and proinflammatory cytokines in wild-type and RNF167-deleted cells infected with SeV for 8 h (n = 3). c qPCR and immunoblot analysis of RNF167 expression levels in THP-1 cells transfected with siRNAs targeting RNF167 or the negative control non-targeting siRNA (siCtrl) (n = 3). d qPCR analysis of the mRNA expression levels of IFNB, the related ISGs and proinflammatory cytokines in THP-1 cells transfected with these siRNAs for 48 h and then infected with VSV for another 8 h (n = 3). e Dual-luciferase assays of the indicated promotor reporters in WT and KO (RNF167-deletion) cells transfected with RNF167-HA expression plasmid or its empty vector as control for 24 h and then infected with SeV for another 8 h. The expression of RNF167 was verified by immunoblot (n = 3). f Representative images of microscopy analysis in wild-type and RNF167-deleted cells infected with VSV-GFP for 24 h at the MOI of 0.1 (scale bars, 50 µm), and the VSV-GFP replication levels were presented in a graph by counting the infected cells (n = 3). g Virus titer detection by plaque assay of the cell supernatant in WT and RNF167-deletion cells infected with VSV-GFP in the MOI of 0.1 for 24 h (n = 3). Data are representative of three independent experiments with similar results, and are shown as mean with SD (a–g). The unpaired two-tailed t-test was applied. GAPDH or Actin were used as a loading control. Source data are provided as a Source Data file.