Fig. 3: RNF167 inhibits the production of type I interferon and antiviral response in vitro. | Nature Communications

Fig. 3: RNF167 inhibits the production of type I interferon and antiviral response in vitro.

From: RNF167 mediates atypical ubiquitylation and degradation of RLRs via two distinct proteolytic pathways

Fig. 3: RNF167 inhibits the production of type I interferon and antiviral response in vitro.

a, b qPCR analysis of the Ifnb1, Cxcl10, Ifit1, Cxcl1, and Il6 mRNA expressions levels in Rnf167+/+ and Rnf167-/- BMDMs/BMDCs infected with SeV and VSV for 8 h (n = 3). c ELISA quantification of IFN-β secretion in Rnf167+/+ and Rnf167-/- BMDMs/BMDCs infected with SeV and VSV for 12 h (n = 3). d qPCR analysis of the Ifnb1, Cxcl10, and Cxcl1 mRNA expression levels in WT/KO BMDMs transfected with the poly(I:C) of high molecular weight (HMW, -H) and low molecular weight (LMW, -L) for 12 h (n = 3). e qPCR analysis of the Ifnb1 induction by SeV infection in Rnf167+/+ and Rnf167-/- BMDMs at the indicated time points (n = 3). f, g Immunoblot analysis of the phosphorylated TBK1 and IRF3 in BMDMs/ BMDCs stimulated by SeV/VSV infection for 8 h, and poly(I:C)-H/L transfection for 12 h. Data are representative of three independent experiments with similar results and are shown as mean with SD (a–e). The unpaired two-tailed t-test was applied. GAPDH was used as a loading control. Source data are provided as a Source Data file.

Back to article page