Fig. 5: RNF167 specially interacts with RIG-I, MDA5 and MAVS.

a Dual-luciferase assays of the indicated promotor activities in HEK293 cells transfected with the empty vector (as control), RIG-I, MDA5, MAVS, TBK1, or IRF3-5D (TRAF6, IKK-β, and p65 for NF-κB-luc) expression plasmids together with RNF167-HA or its empty vector as control for 24 h (n = 3). b Dual-luciferase assays of IFN-β promotor activities in HEK293 cells transfected with negative control (siCtrl) or the siRNA targeting RNF167 (siRNF167) for 24 h, and then transfected with the transfection of empty vector (control), RIG-I, MDA5, MAVS, TBK1, or IRF3-5D expression plasmids for another 24 h (n = 3). c Immunoprecipitation and immunoblot analysis of endogenous RNF167 with RIG-I and MDA5 in HEK293 cells infected with SeV for the indicated time, and the IgG was set as control. d Immunoprecipitation and immunoblot analysis of endogenous RNF167 with IgG (control), MAVS, TBK1, and IRF3 in HEK293 cells infected with SeV for indicated time points. e Schematic structures of human RNF167. SP, signal peptide; PA, protease-associated domain; TM, transmembrane domain, RING domain, and the C terminal domain. f Coimmunoprecipitation and immunoblot analysis of IgG control, Flag-tagged empty vector or Flag-RIG-I with RNF167-HA or its mutants ΔPA/ΔRING/ΔC /ΔR-ΔC (deletion of PA, RING, C, or both RING-C domains) plasmids transfected into HEK293 cells. g Schematic structures of human RIG-I. h Coimmunoprecipitation and immunoblot analysis of IgG control, Flag-tagged empty vector, Flag-RIG-I-CARDs/Helicase/CTD or -ΔCARDs/ΔHelicase/ΔCTD (domains remained and deleted mutants) with RNF167-HA transfected into HEK293 cells. Data are representative of three independent experiments and are shown as mean with SD (a, b). The unpaired two-tailed t-test was applied (ns, not significant). GAPDH was used as the loading control. Source data are provided as a Source Data file.