Fig. 7: DNA extension by Pol θ beyond mismatches.
From: Structural basis of error-prone DNA synthesis by DNA polymerase θ
a, b Extension of a DNA substrate by Pol θ featuring a (a) T:T or (b) T:G mismatch at position -1. c Extension of a DNA substrate by Pol θ featuring a T:T or T:G mismatch at position -2. For all panels, DNA synthesis was conducted with individual nucleotides (A, T, C, or G) or a mix of all four dNTPs (N), with the untreated DNA control denoted as “-''. Product formation (%) is indicated below each gel, and lengh of the primers and the most abundant final products are noted. The DNA sequences and potential base pairings at the primer terminus are depicted on the side of gels. The gels shown here are representatives, with three independent replicates yielding consistent results. Source data are provided as a Source Data file.
