Fig. 3: L1 S-PTGS is delayed when partially impairing CHR11 and CHR17.
From: Arabidopsis SGS3 is recruited to chromatin by CHR11 to select RNA that initiate siRNA production
a GUS activity at 12 days in the progeny of line L1/ pUBQ10:CHR11 #46. Plants exhibiting a wild-type phenotype [wt] (#46.10.7 and #46.26.2) were harvested individually. Box plots show the five-number summary of each set of data: including the minimum score, first (lower) quartile, median, third (upper) quartile, and maximum score. Means are indicated by a cross, and the number of seedlings analyzed is indicated. Plants exhibiting a chr11 chr17 phenotype indicative of CHR11/CHR17 cosuppression (#46.10.7* and #46.26.2*) were pooled to form a unique sample. b GUS and CHR11 siRNA accumulation at 12 days in the progeny of line L1/ pUBQ10:CHR11 line #46. Plants were harvested in bulk. GUS activity is given below for each sample used for RNA extraction (the results are shown for the northern blot obtained from one unique extraction of each sample). c Kinetics of GUS activity in the progeny of lines L1/ pUBQ10:amirCHR11-17 #125 and #135, which exhibit strong CHR11/CHR17 cosuppression. At 7 and 12 days, plants were harvested in bulk (results at 17, 24, and 40 days are shown in Supplementary Fig. 8). d GUS mRNA, GUS siRNA and amiRCHR11-17 accumulation at 18 days in the progeny of lines L1/ pUBQ10:amirCHR11-17 lines #125 and #135 harvested in bulk. GUS activity is given below for each sample used for RNA extraction (the results are shown for the northern blot obtained from one unique extraction of each sample).
