Fig. 4: CHR11 interacts with p35S:GUS transgene at the L1 locus.
From: Arabidopsis SGS3 is recruited to chromatin by CHR11 to select RNA that initiate siRNA production
ChIP-qPCR analyses were performed on 15-day-old seedlings of the indicated genotypes. a ChIP was performed on the L1/pUBQ10:GFP-CHR11 line using GFP antibodies for IP. The L1 line was used as a control, followed by normalization to GAPDH. Graphical representation shows the fold change of two biological repeats. b ChIP was performed on the L1/pUBQ10:FLAG-CHR11 using Flag antibodies for IP. Here, the L1/pUBQ10:GFP-CHR11, which expressed CHR11 at a similar level than in L1/pUBQ10:FLAG-CHR11, was used as a control, followed by normalization to GAPDH. Graphical representation shows the fold change as the mean of three biological repeats and displays every data point. Error bars represent the standard deviation.
