Fig. 5: GUS RNAs associate with SGS3 in the nucleus.
From: Arabidopsis SGS3 is recruited to chromatin by CHR11 to select RNA that initiate siRNA production
RIP analysis of GUS mRNA associated with SGS3 was performed using L1/sgs3 plants complemented by pRDR6:SGS3-NLS-GFP, and L1 line (without TAG) as control for normalization. RT-qPCR was performed to determine the accumulation levels of 5’ terminal part, central part of GUS transcript in the RIP. qPCR Fold enrichment of the immunoprecipitated (IP) was calculated as 2exp(−ΔΔCt [RIP in SGS3-NLS-GFP line/background in L1]). Fold enrichment of the qPCR internal control, PP2A was also calculated and arbitrarily set to 1. The mean values and standard deviation of three independent experiments are shown as every data point.
