Fig. 2: Visual function and histological features of the retina in ocular-selective Pnpla6-deficient mice.
From: PNPLA6 regulates retinal homeostasis by choline through phospholipid turnover
a Protocol for ocular-specific Pnpla6 deletion. b Immunostaining for PNPLA6 in retinas from tamoxifen-treated Pnpla6fl/flCreER(−) and Pnpla6fl/flCreER(+) mice. Red, PNPLA6; blue, DAPI; arrow, RPE. Scale bar, 50 μm. c qPCR of Pnpla6 in isolated RPE or brain from Pnpla6fl/flCreER(−) and Pnpla6fl/flCreER(+) mice, with Gapdh or Hprt1 as a control (n = 3 independent animals). d HE staining of Pnpla6fl/flCreER(−) and Pnpla6fl/flCreER(+) retinas; brackets, ONL. Scale bar, 50 μm. e OCT analysis of Pnpla6fl/flCreER(−) and Pnpla6fl/flCreER(+) retinas. Brackets, ONL. f Quantified values in OCT analysis of Pnpla6fl/flCreER(−) and Pnpla6fl/flCreER(+) retinas (n = 4 from e). g TEM images of Pnpla6fl/flCreER(−) and Pnpla6fl/flCreER(+) retinas. Arrowheads, disc structure of photoreceptors. Scale bar, 1 μm. h ERG analysis of Pnpla6fl/flCreER(−) and Pnpla6fl/flCreER(+) mice under a dark condition (150.3 cd.s/m2). i Amplitudes of a-wave and b-wave (n = 4 from h). j Amplitudes of a-wave and b-wave of the ERG under dark conditions (n = 7 independent animals). k qPCR of Pnpla6 in isolated RPE or brain from 3-month-old Pnpla6fl/fl and Best1-CrePnpla6fl/fl mice (n = 3 independent animals). l HE staining of Pnpla6fl/fl and Best1-CrePnpla6fl/fl retinas. Scale bar, 50 μm. m TEM images of Pnpla6fl/fl and Best1-CrePnpla6fl/fl retinas. Arrows, microvilli of RPE; arrowheads, disc structure of photoreceptors. Scale bar, 1 μm. n OCT analysis of ONL thickness in Pnpla6fl/fl and Best1-CrePnpla6fl/fl mice (n = 3 independent animals). o TUNEL staining of Pnpla6fl/fl and Best1-CrePnpla6fl/fl retinas. Blue, DAPI; Red arrow, TUNEL-positive cells. Scale bar, 50 μm. p Amplitudes of a-wave and b-wave of the ERG under a dark condition (150.3 cd.s/m2) (n = 4 independent animals). Data are presented as mean ± SEM and representative of at least two independent experiments. P-values were derived from two-way ANOVA with Tukey’s multiple comparison test (f, j, and n) and two-tailed unpaired-t test (c, i, k, and p). *P < 0.05, **P < 0.01, and ***P < 0.001, versus control mice (f, j, and n). Source data are provided as a Source Data file.
