Fig. 3: apCAFs exhibited a predominant localization around TLS. | Nature Communications

Fig. 3: apCAFs exhibited a predominant localization around TLS.

From: Antigen-presenting cancer associated fibroblasts enhance antitumor immunity and predict immunotherapy response

Fig. 3: apCAFs exhibited a predominant localization around TLS.

A H&E staining of tissue section for spatial transcriptoms. B Abundance estimation of various cell populations in spatial transcriptomics. C Abundance estimation and relationship of tertiary lymphoid structures (TLS) signature and apCAFs signature in spatial transcriptomics. Correlation was evaluated using the two-sided Spearman rank correlation coefficient. D Representative images of multiplex immunofluorescence characterizing the distribution of TLS and apCAFs (green: CD74; red: PDGFRA; white: CD19; purple: CD3). Multiplexed immunofluorescence assays are performed twice on tumor samples following assay optimization. E H&E staining and the abundance estimation and relationship of TLS signature and apCAFs signature in another spatial transcriptomics section from different patient. Correlation was evaluated using the two-sided Spearman rank correlation coefficient. F Representative H&E staining images of tissue sections (left) and the proportion of TLS-positive tumors (right) in high apCAFs group (n = 70) and low apCAFs group (n = 70). Statistical significance was determined using two-sided Chi-square test. Source data are provided as a Source Data file.

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