Fig. 3: Both trophic factors and electrical activity promote synaptic gene expression in NMJ myonuclei. | Nature Communications

Fig. 3: Both trophic factors and electrical activity promote synaptic gene expression in NMJ myonuclei.

From: Single-nuclei sequencing of skeletal muscle reveals subsynaptic-specific transcripts involved in neuromuscular junction maintenance

Fig. 3

a SnRNA-seq was performed on BoTX-injected and Musk knockout (KO) TA muscles and respective controls, and the data were clustered together with the previous nerve cut dataset from Fig. 2. Only myonuclei (~60,000) are depicted in the UMAP. Individual conditions and their controls are visualized as split UMAPs to the right. b Violin plots visualizing the expression of selected NMJ genes in myonuclei after nerve cut, BoTX injection and Musk KO. The violin plots were generated from snRNA-seq data, UMAP is depicted in (a). Body myonuclei from all fiber types were pooled to generate an average expression value. c, d and e Pie charts showing how NMJ transcripts are regulated in NMJ myonuclei after BoTX injection and Musk KO compared to sciatic nerve transection. f Violin plots of NMJ transcripts that show different responses to nerve cut, BoTX injection or Musk KO. Nerve cut and the corresponding controls n = 4, the remaining conditions n = 1. In (b–f) DE-analysis was performed using a Wilcoxon Rank Sum test. All transcripts with a p-val. <0.01 and log2FC ± 0.25 are indicated by *. In (c–e), transcripts were categorized as significantly changed when p-val. <0.01 and log2FC ± 0.25.

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