Fig. 1: Pipeline for discovery of novel asthma-SNP associations based on proximity to μ and examples of μ-SNP colocalization.
From: Enhancer RNA transcription pinpoints functional genetic variants linked to asthma
A Schematic of pipeline and filtering approaches to identify high confidence μ-SNPs associated with asthma. B–C GRO-seq tracks from BEAS-2B cells treated as indicated for 30 min and visualized in the Integrative Genomics Viewer (IGV) genome browser based on counts per million mapped reads (vertical scales). Blue indicates reads annotated to the sense strand while red indicates reads annotated to the antisense strand. The transcription start site (TSS) and direction of transcription are marked by arrows at the top of each screenshot. Magnified regions show the locations of μ originally calculated by Tfit (green) and following manual refinement (gray) relative to the indicated SNP within dynamically regulated enhancers containing high (B) or low (C) confidence μ-SNPs.
