Fig. 3: Pairing dynamics are largely unaffected in absence of BRA-2. | Nature Communications

Fig. 3: Pairing dynamics are largely unaffected in absence of BRA-2.

From: Overlapping and separable activities of BRA-2 and HIM-17 promote occurrence and regulation of pairing and synapsis during Caenorhabditis elegans meiosis

Fig. 3: Pairing dynamics are largely unaffected in absence of BRA-2.The alt text for this image may have been generated using AI.

a Schematic representation of the C. elegans germline with indicated Prophase I stages and zones division employed for FISH quantification. Charts show paring quantification of the indicated chromosomes in WT and bra-2(ok1171) mutants. Scale bars report S.E.M., and asterisk denotes statistical significance as calculated by Χ2 test (two-sided, CI. = 0.05, p values zone 2-5: Ch. III – 0.000059, 0.009, 0.000374, 0.000026; Ch. V - <0.00001, 0.000018, 0.000108, 0.007386; Ch. X – 0.004118, 0.14855, 0.176575, 0.018383; Ch. IV (zone 1-5) – 0.00001, 0.00003, 0.000012, 0.003951, 0.00072). At least three germ lines for each genetic background were used for the quantifications. The following number of nuclei was analyzed in WT and bra-2(ok1171), respectively: Ch. III - zone 1 (142, 188), zone 2 (169, 167), zone 3 (150, 166), zone 4 (148, 130), zone 5 (123, 102); Ch. IV - zone 1 (145, 164), zone 2 (122, 178), zone 3 (95, 153), zone 4 (84, 117), zone 5 (57, 114); Ch. V - zone 1 (143, 188), zone 2 (169, 167), zone 3 (152, 168), zone 4 (147, 131), zone 5 (123, 102); Ch. X - zone 1 (150, 261), zone 2 (153, 231), zone 3 (129, 221), zone 4 (106, 197), zone 5 (94, 163). b Mid-pachytene nuclei displaying indicated probes signal (Ch. III, red - Ch. V, green – Ch. IV, red - DAPI in blue) and antibodies (SYP-1, magenta - HIM-8, yellow – DAPI, cyan). Scale bar 5 μm. Analysis was performed in biological triplicates. c Quantification of chromosome movement indexes in live animals assessed by tracking SUN-1::GFP in untreated HA::AID::bra-2; TIR1::mRuby worms and after auxin exposure. Bars indicate ±SD with mean. Statistical analysis was performed by two-tailed Mann-Whitney test (ns= non-significant, *p = 0.02, **p = 0.004). The number of nuclei analyzed was 13 and 20 (-auxin and +auxin, respectively). The experiment was per formed in biological triplicates. d Early-pachytene nuclei showing anti-SYP-1 (yellow) and FISH probe labeling Ch. III (red) and (e) anti-SYP-1 (magenta) and FISH probe labeling Ch. V (yellow). Analysis was performed in biological triplicates. Scale bar 5 μm.

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