Fig. 4: Kmea at site 125 of CypA increased ROS level in HeLa cells.
From: Genetically encoding ε-N-methacryllysine into proteins in live cells
a–c HeLa cells were transfected with CypA-WT-mCherry or CypA-K125MeaK-mCherry, which were either treated with/without 4 µM CsA for 36 h. ROS was measured by flow cytometry. Fluorescence intensity of FITC channels was detected after selection of the same number of CypA+ cells by ECD channel. d Quantitative comparison of ROS levels in different cells. n = 3 independent experiments. ns, not significant; *p < 0.05; **p < 0.01, p = 0.0402 and 0.0048, respectively; (two-tailed t-test/One-way ANOVA analysis). Error bar represents ±one standard deviation. e Model showing CypA 125Kmea modification increases ROS level of cells and it blocks the suppression of CsA on CypA.
