Table 1 Site-directed mutagenesis primers
From: The ER-phagy receptor FAM134B is targeted by Salmonella Typhimurium to promote infection
Mutation | Forward | Reverse |
|---|---|---|
FAM134B LIR Mutant | GATGACGCTGCAGCAGCTGACCAGTCAGAGCTGGATCAAATTGAGAGTGAATTGGGACT | TCAGCTGCTGCAGCGTCATCACCTTCTTCAGTGTCTGTGTCCTCTTCTGGGATGGG |
FAM134B G216R | CATTCCTAGGGTTATACTCAGCTATCTACTGTTACTGTGTGCATT | TGAGTATAACCCTAGGAATGTAACTTCCCAAGATCGTAAAAAATGTG |
SopF E325A | TTATATAGCGGCTCATATTCATGGTGATGTATGTTTATTCAGAG | ATATGAGCCGCTATATAATTCCCTTCATAGCCTTTACCA |
Sopf Y224A | CCAATTGCTGCTGCACTGGACTTTCTGAACGGTG | GCAGCAGCAATTGGTCTGCTTGTAGTGCTAAAAGTTCT |
Sopf Y240A | GGAGGTGCCAGCGCCGCTGGGAAATCATTTTTTG | CGCTGGCACCTCCATTTTCACCGTTCAGAAAGTCC |
