Fig. 1: Structural determination of human LYCHOS.
From: Structural basis for cholesterol sensing of LYCHOS and its interaction with indoxyl sulfate
a Representative size exclusion chromatography (SEC) profile of LYCHOS solubilized in 0.005% LMNG and 0.0005% CHS. The indicated fractions were subjected to SDS-PAGE analysis, resolving multiple bands with different glycosylation states after Coomassie blue staining. Peak fractions were concentrated for cryo-EM sample preparation. The experiments were independently repeated more than three times with similar results. b Two perpendicular views of the final reconstructed LYCHOS. LYCHOS is a dimer with two protomers, MonoA and MonoB. c Two perpendicular views are presented to show the LYCHOS structures. Top: MonoA and MonoB are rainbow- and gray-colored, respectively. Bottom: MonoA is domain-colored with marine, light blue, and wheat for scaffold, transporter, and GPCR domains, respectively, and MonoB is gray-colored. The same color code is applied to all figures unless otherwise indicated.
