Fig. 2: CeMHtr2a and CeMSst neurons promote water intake. | Nature Communications

Fig. 2: CeMHtr2a and CeMSst neurons promote water intake.

From: Food and water intake are regulated by distinct central amygdala circuits revealed using intersectional genetics

Fig. 2

a Viruses injected and optic fiber placement in the CeA (modified from Allen Mouse Brain Atlas, mouse.brain-map.org). Representative images of ChR2-EYFP expression in CeA subregions after injections of INTRSECT Con/Fon or Con/Foff viruses into Htr2a-Cre;Wfs1-FlpoER (b) or Sst-Cre;Wfs1-FlpoER mice (c). All mice used for behavior showed similar results. d, e Similar manipulations as in (b, c) except for INTRSECT expression of EYFP protein. f Water intake during photoactivation of all CeAHtr2a or CeASst neurons for 30-min by water-deprived mice compared to photoactivated controls (Htr2a: unpaired two-tailed t test p < 0.0001, t = 6.429. Sst: Mann-Whitney two-tailed U test p = 0.0124, U = 16). (CeAHtr2a: n = 9 Ctrl, n = 11 ChR2. CeASst: n = 8 Ctrl, n = 12 ChR2 mice). g Water intake during photoactivation of the CeL (Htr2a: unpaired two-tailed t test p = 0.0913, t = 1.774. Sst: unpaired two-tailed t test p = 0.7017 t = 0.3886). (CeLHtr2a: n = 10 Ctrl, n = 12 ChR2. CeLSst: n = 12 Ctrl, n = 10 ChR2 mice). h Water intake during photoactivation of the CeM (Htr2a: Mann-Whitney two-tailed U test p < 0.0001, U = 2. Sst: unpaired two-tailed t test p < 0.0001 t = 5.054). (CeMHtr2a: n = 11 Ctrl, n = 9 ChR2. CeMSst: n = 10 Ctrl, n = 11 ChR2 mice). i–l Photoactivation of CeMHtr2a (i) or CeMSst neurons (j) during alternating 10-min light OFF and ON epochs in water-deprived mice increased water intake during light ON epochs (Htr2a: 10–20 min: Mann-Whitney two-tailed U test p = 0.0002, U = 0. 30–40 min: unpaired two-tailed t test p = 0.0003, t = 4.801. 50–60 min: Mann-Whitney two-tailed U test p = 0.0030, U = 5.500. Sst: 10–20 min: unpaired two-tailed t test p = 0.0002, t = 4.738. 30–40 min: Mann-Whitney two-tailed U test p < 0.0001, U = 1.500. 50–60 min: Mann-Whitney two-tailed U test p = 0.0012, U = 8). Photoactivation of the CeL fractions (k, l) had no effect (Htr2a: 10–20 min: unpaired two-tailed t test p = 0.6180, t = 0.5069. 30–40 min: Mann-Whitney two-tailed U test p = 0.6326, U = 48. 50–60 min: Mann-Whitney two-tailed U test p = 0.4624, U = 49. Sst: 10–20 min: unpaired two-tailed t test p = 0.1053, t = 1.697. 30–40 min: Mann-Whitney two-tailed U test p = 0.4264, U = 48. 50–60 min: Mann-Whitney two-tailed U test p = 0.0697, U = 36). (CeMHtr2a: n = 7 Ctrl, n = 9 ChR2. CeMSst: n = 9 Ctrl, n = 10 ChR2. CeLHtr2a: n = 10 Ctrl, n = 11 ChR2. CeLSst: n = 12 Ctrl, n = 10 ChR2 mice). Values = Mean ± SEM. Scale bar: 115 μm.

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