Fig. 3: CeMHtr2a and CeMSst neurons are required for normal water intake. | Nature Communications

Fig. 3: CeMHtr2a and CeMSst neurons are required for normal water intake.

From: Food and water intake are regulated by distinct central amygdala circuits revealed using intersectional genetics

Fig. 3

a Representative images showing expression of Cre-dependent eNpHR3.0 and control mCherry in the entire CeA of Htr2a-Cre and Sst-Cre animals. All mice used for behavior showed similar results. b Representative images showing expression of IC++ and control YFP predominantly in the CeM after injection of Con/Foff virus in Htr2a-Cre;Wfs1-FlpoER and Sst-Cre;Wfs1-FlpoER mice. All mice used for behavior showed similar results. c Photoinhibition of the entire populations of CeAHtr2a (blue) or CeASst neurons (green) in water-deprived mice significantly decreased water consumption compared to photoinhibited controls during a 30-min drinking assay (Htr2a: unpaired two-tailed t test p = 0.0007, t = 3.939. Sst: unpaired two-tailed t test p = 0.0363, t = 2.213). (CeAHtr2a: n = 12 Ctrl, n = 12 eNpHR3.0. CeASst: n = 13 Ctrl, n = 14 eNpHR3.0 mice). d Photoinhibition of the CeM fractions of CeAHtr2a (blue) or CeASst neurons (green) significantly decreased water consumption compared to photoinhibited controls in the same assay (Htr2a: unpaired two-tailed t test p = 0.0022, t = 3.578. Sst: unpaired two-tailed t test p = 0.0017, t = 3.733). (CeMHtr2a: n = 12 Ctrl, n = 12 IC++. CeMSst: n = 11 Ctrl, n = 9 IC++ mice). Values = Mean ± SEM. Scale bar: 115 μm.

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