Fig. 4: Distinct roles of CeM^Htr2a and CeM^Sst neurons in feeding behavior.

a Photostimulation of the entire population of CeA^Htr2a neurons promoted feeding of satiated mice (two-tailed Wilcoxon signed-rank test p = 0.0078). No statistically significant effect after photostimulation CeA^Sst neurons compared to controls (Mann-Whitney two-tailed U test p = 0.1821, U = 22). (CeA^Htr2a: n = 8 Ctrl, n = 8 ChR2. CeA^Sst: n = 7 Ctrl, n = 10 ChR2 mice). b Photostimulation of CeL^Htr2a or CeL^Sst subpopulations did not promote feeding behavior (CeL^Htr2a: Mann-Whitney two-tailed U test p = 0.1073, U = 30.50. CeL^Sst: p = 0.9183, U = 48). (CeL^Htr2a: n = 10 Ctrl, n = 10 ChR2. CeL^Sst: n = 11 Ctrl, n = 9 ChR2 mice). c Photostimulation of CeM^Htr2a, but not CeM^Sst neurons, increased food intake (CeM^Htr2a: Mann-Whitney two-tailed U test p = 0.0235, U = 19.50. CeM^Sst: p = 0.2783, U = 41.50). (CeM^Htr2a: n = 10 Ctrl, n = 9 ChR2. CeM^Sst: n = 10 Ctrl, n = 11 ChR2 mice). d Photoinhibition of the entire population of CeA^Htr2a neurons in fed mice decreased the ingestion of a palatable liquid reward compared to controls (unpaired two-tailed t test p = 0.0170, t = 2.593). No effect by photoinhibition of the entire population of CeA^Sst neurons (Mann-Whitney two-tailed U test p = 0.1285, U = 37). (CeA^Htr2a: n = 12 Ctrl, n = 11 eNpHR3.0. CeA^Sst: n = 14 Ctrl, n = 14 eNpHR3.0 mice). e Photoinhibition of CeM^Htr2a, but not CeM^Sst, neurons decreased the consumption of a palatable liquid reward compared to controls (CeM^Htr2a unpaired two-tailed t test p = 0.0005, t = 4.313; CeM^Sst unpaired two-tailed t test p = 0.5433, t = 0.6230). (CeM^Htr2a: n = 9 Ctrl, n = 10 IC + +. CeM^Sst: n = 10 Ctrl, n = 6 IC + + mice). Values = Mean ± SEM.