Fig. 2: PLX5622 treatment promotes remyelination and angiogenesis after ischemic stroke. | Nature Communications

Fig. 2: PLX5622 treatment promotes remyelination and angiogenesis after ischemic stroke.

From: Repair-associated macrophages increase after early-phase microglia attenuation to promote ischemic stroke recovery

Fig. 2

A, B UMAP projection plot (A) and stacked bar plot (B) showing cell clusters harvested from the ipsilateral brain hemisphere at 14 dpi. n = 2 biological replicates for each group. C, D Representative images and quantification for MBP (green) and SMI32 (red) double immunostaining in the peri-infarct areas at 14 dpi. n = 6 per group. Scale bars, 200 µm (Cortex), and 100 µm (Striatum and External Capsule). Data were normalized to the intensities of contralateral hemispheres. One-way ANOVA and Tukey. E UMAP projection of oligodendrocytes. F Pseudotime trajectory of oligodendrocyte subclusters. G Violin plot illustrating the expression of Klk6 and Cntn1 in oligodendrocyte (up). Stacked bar plot showing the proportion of OLG in (E) (down). H UMAP projection of OPCs. I Doughnut plot showing an increase of OPCs in the G2M phase in the PLX5622-treated mice. J Representative images and quantification for APC (green) and BrdU (red) double immunostaining in the peri-infarct areas at 14 dpi. n = 5 per group. K Box plot showing the upregulation of genes related to angiogenesis in EC. n = 557 ECs for control group and n = 1057 ECs for PLX5622 group, from two biological replicates. Wilcoxon rank sum test, two-sided. Box plot represents the first quartile, median, and third quartile with whiskers extending to 1.5 times the interquartile range. L Representative images and quantification for CD31 (green) and BrdU (red) double immunostaining in the peri-infarct areas at 14 dpi. n = 6 per group. J, L Student’s t test, two-sided. Data in (D, J, L) are presented as mean ± SD. MG microglia, MF macrophages, BAMs border-associated-macrophages, DC dendritic cells, NP neutrophils, NK natural killer cells, γδT gamma delta T cells, NEUB neuroblasts, ASC astrocytes, OLG oligodendrocytes, OPC oligodendrocyte progenitor cells, EC endothelial cells, PC pericytes, FB fibroblast-like cells, EPC ependymocytes, CPC choroid plexus epithelial cells, OEG olfactory ensheathing glial cells. Source data are provided as a Source Data file.

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