Fig. 2: Calcium imaging reveals the functional activity of forebrain organoids.
From: Astrocyte-secreted cues promote neural maturation and augment activity in human forebrain organoids
a Generation and air-liquid culture of forebrain organoid slices for calcium imaging (Created in BioRender. Yang, H. (2025) https://BioRender.com/r36w725). b Sample images of Fluo-4 AM of a forebrain organoid and acquisition of regions of interests (ROIs) illustrating the identification of active neurons. Activity profiles of all active neurons are shown as normalized ΔF/F values and colorized amplitude plots. Activities of one spiking neuron (blue) and a multi-spiking neuron (red) are identified and visulaized. Scale bar, 100 μm. c Calcium imaging of MACMOs on days 45, 90, and 120. Activity profile is shown as normalized ΔF/F values and colorized amplitude plot. The experiments were repeated at least three times independently with similar results. The calcium imaging of forebrain organoids cultured under control conditions is shown in Supplementary Fig. 6.
