Fig. 5: MACMOs develop more spontaneous network activity in the long-term culture via MEA recoding.
From: Astrocyte-secreted cues promote neural maturation and augment activity in human forebrain organoids
a Schematic representation of the electrical activity feature plots from the MEA recording software (Created in BioRender. Yang, H. (2025) https://BioRender.com/q32h063). b Example spike raster plots from one well, raw traces, and spike waveform, recorded from single electrodes in connection with MACMOs and control organoids at the early stage (days 45–60) and the late stage (days 61–90). Quantification of electrophysiological activity of the number of (c) spikes, (d) bursts, (e) network bursts, (f) active electrodes, (g) bursting electrodes, and (h) electrodes participating in bursts from forebrain organoids with MEA recording in 5 minutes. The data are represented as  mean ± SEM. Each dot represents 5-min recording from one organoid. N = 27 and 99 recordings at early stages and late stages for each condition, respectively; Significant difference is determined by two-tailed Mann–Whitney U test; *P  <  0.05, **P  <  0.01, ***P  <  0.001; ****P  <  0.0001. Source data are provided as a Source Data file.
