Fig. 7: ACM culture elevates lipid droplet levels in neurons and astrocytes of organoids.
From: Astrocyte-secreted cues promote neural maturation and augment activity in human forebrain organoids
GSEA of GO terms related to the lipid metabolism in astrocytes (a) and radial glial cells (b) between control and ACMOs. c Sample confocal images of day-60 ACMOs and control organoids, for immunostaining of LipidSpot488, GFAP, and TUJ1 at day 60. Scale bar, 20 μm. d, g Quantification of the number and average size of lipid droplets per field in organoids. e, f, h, i Quantification of the number and average size of lipid droplets per field colocalized with TUJ1+ or GFAP+ cells in organoids. The data are represented as mean ± SEM. Each point represents one region of interest of one day 60 organoid section. N = 9 replicates for each condition; Significant difference is determined by two-tailed unpaired t test for (e, g–i); two-tailed unpaired t test with welch correction for (d); two-tailed Mann–Whitney U test for (f). *P  <  0.05, **P  <  0.01, ***P  <  0.001. a–c are created in BioRender. Yang, H. (2025) https://BioRender.com/p79r180. Source data are provided as a Source Data file.
