Fig. 8: MACM and HACM increase the lipid droplet level during NPCs and forebrain neurons differentiation.
From: Astrocyte-secreted cues promote neural maturation and augment activity in human forebrain organoids
a Schematic diagram illustrating the treatment of NPCs and forebrain neurons with different culture media, followed by immunocytochemistry. b, e Sample confocal images of NPCs or forebrain neurons, for immunostaining of lipid droplets marker, LipidSpot; astrocyte marker, GFAP; neuron marker, TUJ1. Scale bar, 20 μm. c, d, f, g, h Quantitative analysis of lipid droplets number and the number of lipid droplets colocalized with GFAP+ or TUJ1+ cells. The data are represented as  mean ± SEM. N = 9 replicates for each condition; The significant difference is determined by one-way ANOVA, followed by Tukey’s test. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. a, b and e are created in BioRender. Yang, H. (2025) https://BioRender.com/j55r024. Source data are provided as a Source Data file.
