Fig. 1: Crystal cell differentiation increased in Atg1Δ3D mutants.
Confocal images of individual lobes of larval lymph glands; primary lobes indicated in white dashed lines. In Atg1Δ3D homozygous mutants, autophagy activation was reduced, as indicated by 3xmCherry-Atg8a nucleation (A n = 10 primary lobes for wild type, n = 8 for Atg1Δ3D; p < 0.0001). Progenitor (domeMESO > GFP; B n = 13 for wild type, n = 14 for Atg1Δ3D) or plasmatocyte (P1; C n = 24 for wild type, n = 28 for Atg1Δ3D) populations were unaffected in Atg1 mutants (p = 0.2210; p = 0.8221, respectively). Crystal cells increased in Atg1Δ3D mutant larvae as assessed by either anti-Lozenge (Lz; D n = 7 for each genotype; p = 0.0009) or anti-prophenoloxidase (PPO; E n = 10 for each genotype; p = 0.0016). Box plots were used to visualize data distribution, where the box represents the interquartile range (25th and 75th percentiles), the central line indicates the median, and whiskers indicate the minimum and maximum values in the dataset. Individual data points are shown. Statistical analysis was performed using an unpaired two-tailed Student’s t-test. **p < 0.01; ***p < 0.001; ****p < 0.0001; ns, not significant (p > 0.05). Source data for plots are provided as a Source Data file.
