Fig. 2: Knock-down of autophagy pathway genes in blood cell progenitors provoked increased crystal cell differentiation. | Nature Communications

Fig. 2: Knock-down of autophagy pathway genes in blood cell progenitors provoked increased crystal cell differentiation.

From: Autophagy controls differentiation of Drosophila blood cells by regulating Notch levels in response to nutrient availability

Fig. 2: Knock-down of autophagy pathway genes in blood cell progenitors provoked increased crystal cell differentiation.The alternative text for this image may have been generated using AI.

Crystal cells were visualized by anti-Lozenge (Lz) or anti-prophenoloxidase (PPO) immunofluorescence in Z-stack projections of lymph gland lobes (white dashed lines) where the indicated double-stranded RNAs, affecting different autophagy genes, have been expressed under control of a domeMESO-Gal4 driver (A–F). Crystal cell differentiation increased in comparison to wild type controls (UAS-LacZ). Quantification of the normalized number of crystal cells per lobe is depicted on panel (G) where box plots show the data distribution, with the box representing the interquartile range, the central line indicating the median, and whiskers extending to the minimum and maximum values. For Lz+ cells, statistical analysis was performed using two-sided likelihood ratio test (Chi-squared) followed by Dunnett’s test for treatment versus control comparisons (p < 0.0001; p < 0.0001; p = 0.0862; p < 0.0001; p = 0.0010, respectively). For PPO+ cells, ANOVA followed by Dunnett’s test for treatment versus control comparisons was used (p < 0.0001; p < 0.0001; p = 0.0002; p < 0.0001; p < 0.0001, respectively). For wild type, n = 65 primary lobes; for Atg1RNAi, n = 24; for Atg17RNAi, n = 25; for Vps15RNAi, n = 19; for Vps34RNAi, n = 13; for Atg18RNAi, n = 21 for Lz, n = 20 for PPO. Source data for plots are provided as a Source Data file. ***p < 0.001; ****p < 0.0001; ns, not significant (p > 0.05).

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