Fig. 7: Blockage of lysosomal maturation or impairment of fusion events that give rise to amphisomes or autolysosomes increased crystal cell differentiation. | Nature Communications

Fig. 7: Blockage of lysosomal maturation or impairment of fusion events that give rise to amphisomes or autolysosomes increased crystal cell differentiation.

From: Autophagy controls differentiation of Drosophila blood cells by regulating Notch levels in response to nutrient availability

Fig. 7

Anti-Lozenge (Lz) and anti-prophenoloxidase (PPO) staining were used to visualize crystal cells in Z-stack projections of the lymph gland (white dashed line). After knock-down of the V-ATPase subunit Vha44 or the lipid kinase Fab1 with a domeMESO-Gal4 driver, an increase of crystal cell differentiation occurred in comparison with wild type control larvae (A–C). Knock-down of the SNAREs Vamp7 or Syntaxin-17 also led to augmented crystal cell differentiation (D, E). F Quantification of normalized crystal cells per lobe, as assessed with anti-Lz and anti-PPO antibodies respectively. Box plots show the data distribution, with the box representing the interquartile range, the central line indicating the median, and whiskers extending to the minimum and maximum values. Wild type, n = 22; Vha44RNAi, n = 24; fab1RNAi, n = 21; Vamp7RNAi, n = 20; Syx17RNAi, n = 19. Brown-Forsythe and Welch ANOVA followed by Dunnett’s T3 test for treatments against control comparisons (for Lz, p = 0.0037, p < 0.0001, p < 0.0001, p < 0.0001, respectively; for PPO, p < 0.0001, p = 0.0209, p = 0.0002, p = 0.0013, respectively). Source data for plots are provided as a Source Data file. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.

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