Fig. 1: PDHA1 K83 is highly succinylated in cholangiocarcinoma and closely related to survival. | Nature Communications

Fig. 1: PDHA1 K83 is highly succinylated in cholangiocarcinoma and closely related to survival.

From: Cholangiocarcinoma PDHA1 succinylation suppresses macrophage antigen presentation via alpha-ketoglutaric acid accumulation

Fig. 1

a Upregulated genes revealed by succinylation proteomic analysis of cholangiocarcinoma and adjacent non-tumor tissues (n = 10). b Mass spectrometry identified the succinylation modification site K83 on PDHA1. c Quantification of PDHA1 K83Succ immunostaining performed to compare paired samples (n = 74). d Kaplan–Meier analysis of OS curves based on PDHA1 K83Succ expression. PDHA1 K83Succ expression associated with poor prognosis in CCA patients. e Representative immunofluorescence staining of CCA and adjacent normal tissues (n = 7). PDHA1 K83Succ (Red), DAPI (Blue). And statistical chart of PDHA1 K83Succ mean gray values. Scale bars: 50 μm. f Schematic illustrating transposon- and CRISPR-Cas9-(p53)-based injection of vectors into mice via the tail vein to generate tumors resembling human CCA. The transposon-based vector overexpressing KrasG12D can express luciferase. Created in BioRender. Zhang, N. (2025) https://BioRender.com/y78d999. Bioluminescence images and signal of C57BL/6 mice after plasmid injection on day 7 and day 28 (n = 10 biologically independent samples). g Front and back representative images of livers from (f). Scale bars: 1 cm. h Kaplan–Meier survival curve of C57BL/6 mice treated as in (f). i Tumor incidence of C57BL/6 mice treated as in (f). j Schematic illustrating transposon- and CRISPR-Cas9-(Pten)-based injection of vectors into mice via the tail vein to generate tumors resembling human CCA. The transposon-based vector overexpressing KrasG12D can express luciferase. Created in BioRender. Zhang, N. (2025) https://BioRender.com/j69i397. Bioluminescence images and signal of C57BL/6 mice after plasmid injection on day 7 and day 28 (n = 8 biologically independent samples). k Front and back representative images of livers from (j). Scale bars: 1 cm. l Kaplan–Meier survival curve of C57BL/6 mice treated as in (j). m Tumor incidence of C57BL/6 mice treated as in (j). Data are presented as mean values ± SD, and P values were calculated using two-tailed unpaired Student’s t-tests (c, e, f, j) or log-rank test (d, h, l). HR hazard ratio, WT wild type. Source data are provided as a Source Data File.

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