Fig. 1: Workflow of genome-scale CRISPR knockout (GeCKO) screens in THP-1 cells. | Nature Communications

Fig. 1: Workflow of genome-scale CRISPR knockout (GeCKO) screens in THP-1 cells.

From: Genetic modulation of rare earth nanoparticle biotransformation shapes biological outcomes

Fig. 1: Workflow of genome-scale CRISPR knockout (GeCKO) screens in THP-1 cells.The alternative text for this image may have been generated using AI.

This schematic illustrates the methodology employed for genome-scale CRISPR knockout screens in THP-1 cells, including construction of CRISPR-Cas9 plasmid library and cell pool, La2O3 treatment, gDNA extraction, sgRNA amplification and sequencing. Some image elements were adapted from figures provided by Servier Medical Art, licensed under a Creative Commons Attribution 4.0 Unported License (https://creativecommons.org/licenses/by/4.0/).

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