Fig. 5: Validation of SMPD1 regulation on the biotransformation and adverse effects of La2O3 in animal lungs. | Nature Communications

Fig. 5: Validation of SMPD1 regulation on the biotransformation and adverse effects of La2O3 in animal lungs.

From: Genetic modulation of rare earth nanoparticle biotransformation shapes biological outcomes

Fig. 5: Validation of SMPD1 regulation on the biotransformation and adverse effects of La2O3 in animal lungs.The alternative text for this image may have been generated using AI.

a Western blot analysis of ASM expression, the representative images were selected from 3 independent experiments with similar results. b TEM visualization of La2O3 in alveolar macrophages from bronchoalveolar lavage fluid (BALF). Blue arrows show the representative nanoparticles in cells. Quantification of (c) IL-1β and (d) TGF-β1 in BALF, bar plots represent means with SDs (n = 6 biologically independent mice), ***p < 0.001 compared with the mice without treatment of desipramine (DPM) by one-way ANOVA test. e H&E staining and (f) Masson trichrome staining of lung tissues. Mice pretreated with or without DPM were exposed to La2O3 via oropharyngeal aspiration (n = 6 mice for each treatment). Alveolar macrophages were obtained from BALF. Scale bars in (e and f) represent 150 μm. Source data are provided as a Source Data file.

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