Fig. 4: SAT1 depletion reduced H3K27ac in mitosis-regulating genes.

a Western blot analysis of common histone modifications in cells with or without SAT1 knockdown under detachment for 12 h. Histone H3 or histone H4 was used as a loading control. b, c Average profile of H3K27ac ChIP peaks binding to the TSS region (b) and annotation of the location of H3K27ac peaks in terms of genomic features (c) in detached cells with or without SAT1 knockdown via ChIPseeker tools (n = 3, each). GEO accession number of the ChIP-Seq data is GSE267703. d Venn diagram showing the overlap between genes with reduced expression and genes with reduced H3K27ac deposition in detached SAT1-depleted cells compared with pLKO control cells (n = 3, each). e Analysis of the top GO terms associated with the biological processes associated with the overlapping differentially expressed genes using clusterProfiler tool. Adjusted P values are shown. f Cnetplot visualizing the genes that are involved in the significantly enriched biological process terms using clusterProfiler tools. Adjusted P values are shown. g ChIP‒qPCR analysis of H3K27ac enrichment in several key genes identified in (f) and related to the mitotic cell cycle process, chromosome organization, and chromosome segregation in detached cells with or without SAT1 knockdown. The data are representative of 2 independent biological experiments for (a) and are presented as the means ± SDs from 3 biological replicates for (g). Statistical analysis was performed via two-tailed Student’s t test for (G). The samples derive from the same experiment but different gels for all indicated histone antibodies were processed in parallel for (a). See also supplementary Figs. S5 and S6.