Fig. 2: Spatial transcriptomic atlas of the brain sections of nurse and forager bees revealed subclusters in anatomical regions. | Nature Communications

Fig. 2: Spatial transcriptomic atlas of the brain sections of nurse and forager bees revealed subclusters in anatomical regions.

From: Single-nucleus and spatial transcriptomics identify brain landscape of gene regulatory networks associated with behavioral maturation in honeybees

Fig. 2: Spatial transcriptomic atlas of the brain sections of nurse and forager bees revealed subclusters in anatomical regions.

a A schematic view of the honeybee brain with the main structures, including the mushroom body (MB), ocelli (OC), vertical lobe (VL), antennal lobe (AL), lobula (LO), medulla (ME), lamina (LA), and retina (RE). b Hematoxylin-eosin staining of one of the representative brain sections of the honeybee. c Nucleic-acid dye staining of the brain section shown in (b) attached on the Stereo-seq chip. Unsupervised clustering of the brain sections from nurse (d) and forager (e) bees based on the Stereo-seq data at bin-50 (50 × 50 DNB) resolution. Bins are colored based on the annotation of the clustering. Projection of our snRNA-seq data onto the brain sections of nurse (f) and forager (g) bees using Tangram. The color represents the relative probability of each cell type being mapped to the specific locations within the sections. Scale bars, 0.3 mm.

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