Fig. 4: Subclustering of optic lobe cells from honeybee worker brains.
a tSNE visualization for the subclustering of OLCs from the worker brains, which are grouped into eight subtypes. Cluster identifiers from Seurat clustering at a resolution of 0.2 are indicated in parentheses. b Correspondence of OLC clusters between Apis mellifera, Monomorium pharaonis35, and Drosophila melanogaster40. The lines with different thickness link OLC clusters from A. mellifera with those from M. pharaonic and D. melanogaster according to the AUROC scores. Source data are provided as a Source Data file. c A schematic diagram of the structural characteristics of neurons from the optic ganglia of the honeybees (adapted from Ribi and Scheel23). svf, short visual fibers; lvf, long visual fibers; L, L-fibre; Tnf, tangential fiber with narrow-field ending; Twf, tangential fiber with wide-field ending; Tm, transmedullary neuron. Spatial visualization of the distribution of the OLC4 subcluster in the optic lobe regions of the brain sections of nurse (d) and forager (e) bees inferred by Tangram. The color of each merged bin is scaled according to the Tangram scores based on the projection of the snRNA-seq dataset. Scale bars, 0.3 mm.
