Fig. 6: Identification of new Notch signaling interactions with size effect on the excretory cell progenitors and a symmetric cell.
A Lineal expression (blueness on cell lineage tree) of two ligands, lag-1 and apx-1, and one receptor, lin-12, of the Notch signaling pathway, each derived from an exemplary wild-type embryo. The lag-2 expression in MSapap is deduced based on previous studies43,75,84,85. The color scale is shown on the right. Cells involved in the sixth-tenth signaling events are indicated with arrows. Apoptosis is marked with an “X”. B Reconstructed 3D morphologies of contacting cell pairs engaged in the newly identified Notch signaling events during C. elegans embryogenesis. Cells expressing Notch ligands are highlighted in green, while those expressing the receptor are in red. C Comparison of morphological changes between the Notch-responsive ABprpapp cell (2nd row) that is deduced to receivs the tenth Notch signaling and its sibling (1st row), which is deduced to receive substantially weaker or no effective Notch signaling, in a wild-type embryo, or the ABprpapp cell in a perturbed embryo by RNA against lag-1 (3rd row) or laser ablation on MSpp (4th row). Note the directional size asymmetry in the division of ABprpapp (2nd row) in contrast to its sister (1st row), or its perturbed counterpart in embryos with RNAi against lag-1 (3rd row) or laser ablation of MSpp (4th row). \({T}_{{{{\rm{C}}}}}\) denotes the last time point of cytokinesis. D Positions (illustrated with the cell nucleus positions) of the Notch-responsive cells (red), Notch-signaling cells (green), and others (semi-transparent gray) at the moments when the sixth-tenth signaling events take place. Source data are provided as a Source Data file.
