Fig. 2: EP and GPEET levels are regulated by TblncRNA-23.

A Ciros plot showing TblncRNA-23 mRNA chimeric molecules. The data are from Rajan et al.²². B (i) TblncRNA-23 silencing. Cells were silenced for indicated days and the RNA was subjected to Northern analysis. Data from n = 3 experiments are presented as mean ± S.E.M., and p value was determined by Student’s t-test one tailed distribution. (ii) TblncRNA-23 in KO cells. RNA from two independent KO clones was subjected to Northern analysis. C (i) Expression of GPEET following TblncRNA-23 silencing. Lysates from induced ( + TET) and uninduced (-TET) cells were subjected to western analysis with the indicated antibodies (left). The expression level is presented as mean ± S.E.M. The p values are indicated (n = 3) and determined by Student’s t-test one-tailed distribution. ZC3H41 was used as a loading control. (ii) Expression of EP following TblncRNA-23 silencing. Whole cell lysates from induced ( + TET) and uninduced (-TET) cells were subjected to western analysis with the indicated antibodies. The expression level is presented as mean ± S.E.M (n = 3). The p values were determined by Student’s t-test one tailed distribution. PTB2 was used as a loading control. D (i) Cells were silenced for the indicated number of days, and subjected to Northern analysis. The experiments (n = 3) are presented as mean ± S.E.M., and p value was determined by Student’s t-test one tailed distribution. (ii) Northern analysis of EP mRNA in cells silenced for TblncRNA-23. Cells were silenced for the indicated number of days, and RNA was subjected to Northern analysis. The data (n = 3) are presented as mean ± S.E.M. and p value was determined by Student’s t-test one tailed distribution. E, F Expression of GPEET and EP following TblncRNA-23 KO. Extracts from PS and KO cells were subjected to Western analysis and RNA was subjected to Northern analysis. The expression level is presented as mean ± S.E.M (n = 3). The p values were determined by Student’s t-test one tailed distribution. ZCH341 and 7SL RNA were used as loading controls. Source data for all panels are provided in the Source Data file.