Fig. 8: Manipulation of GHS-R1a expression in VMH αCaMKII+ neurons. | Nature Communications

Fig. 8: Manipulation of GHS-R1a expression in VMH αCaMKII+ neurons.

From: GHS-R1a signaling drives anxiety-related behavior by shaping excitability of ventromedial hypothalamic neurons

Fig. 8: Manipulation of GHS-R1a expression in VMH αCaMKII+ neurons.

a Left, flowchart. Created in BioRender. Zhang, M. (2025) https://www.biorender.com/0xmmrx8. Right, reducing endogenous GHS-R1a expression in the VMH by RNA interference in mice at the baseline state. n = 6 mice per group for the EPM test, two-tailed unpaired t-test, t = 3.892, df = 10, **p < 0.01. n = 4 mice per group for Ghsr1a qPCR analyses, two-tailed unpaired t-test, t = 3.695, df = 6, *p < 0.05. b Left, flowchart. Created in BioRender. Zhang, M. (2025) https://www.biorender.com/ntncb6i. Right, Cre-dependent knockdown of GHS-R1a expression in VMH αCaMKII+ neurons in mice at the baseline state. n = 8 mice per group, two-tailed unpaired t-test, t = 3.120, df = 14, **p < 0.01. c Left, flowchart. Created in BioRender. Zhang, M. (2025) https://www.biorender.com/kq0q3vw. Right, Cre-dependent GHS-R1a overexpression in the VMH αCaMKII+ neurons in mice at the baseline state. n = 7 mice per group for the EPM test, two-tailed unpaired t-test, t = 3.134, df = 12, **p < 0.01. n = 3 or 4 mice per group for Ghsr1a qPCR analyses, two-tailed unpaired t-test, t = 2.649, df = 5, *p < 0.05. d Left, flowchart. Created in BioRender. Zhang, M. (2025) https://www.biorender.com/45xcg26. Right, Camk2a-driven GHS-R1a overexpression in the VMH of mice at the baseline state. n = 8 mice per group, two-tailed unpaired t-test, t = 2.336, df = 14, *p < 0.05. e Representative FISH images showing Cre-dependent Ghsr1a knockdown or Cre-independent Ghsr1a overexpression in VMH αCaMKII+ neurons. Scale bar, 10 μm. f Left, flowchart. Created in BioRender. Zhang, M. (2025) https://www.biorender.com/fyd0dwo. Right, the behavioral effect of reduced endogenous GHS-R1a expression in the VMH on RRS-exposed mice. n = 6 mice per group, two-tailed unpaired t-test, t = 4.997, df = 10, ***p < 0.001. g Left, flowchart. Created in BioRender. Zhang, M. (2025) https://www.biorender.com/bq7uss3. Right, the behavioral effect of knockdown of GHS-R1a expression in VMH αCaMKII+ neurons on RRS-exposed mice. n = 7 or 8 mice per group, two-tailed unpaired t-test, t = 2.243, df = 13, *p < 0.05. h Left, flowchart. Created in BioRender. Zhang, M. (2025) https://www.biorender.com/h53m67g. Right, the behavioral effect of GHS-R1a overexpression in VMH αCaMKII+ neurons on RRS-exposed mice. n = 7 mice per group, two-tailed unpaired t-test, t = 3.821, df = 12, **p < 0.01. All bar graphs represent mean ± SEM.

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