Fig. 1: Identification and molecular characterization of the SUG1 gene.
From: A molecular framework for the GS2-SUG1 module-mediated control of grain size and weight in rice
a–c Mature grains (a), plants (b) and panicles (c) of ZH11, ZH11-GS2AA, sug1-1 ZH11-GS2AA, sug1-1 ZH11-GS2AA;proSUG1:SUG1 #1, sug1-1 ZH11-GS2AA;proSUG1:SUG1 #2, and sug1-1 ZH11-GS2AA;proSUG1:SUG1 #3. d The SUG1 gene structure includes untranslated regions (white boxes), exons (black boxes), and introns (lines between boxes). The mutation sites of sug1-1 and sug1-2 are indicated in the diagram. e The schematic diagram of the SUG1 protein and the truncated proteins generated by sug1-1 and sug1-2 mutations. The SUG1 protein comprises a BTB/POZ-like domain, three coiled-coil domains, and a Nuclear localization signal. The mutation in sug1-1 and sug1-2 results in truncated proteins, which lack the C-terminal coiled-coil and nuclear localization signal. f The mutant sites of sug1-1 and sug1-2 were confirmed by sequencing. g–k Grain length (g), grain width (h), 1,000 grain weight (i), plant height (j), and panicle length (k) of ZH11, ZH11-GS2AA, sug1-1 ZH11-GS2AA, and genomic complementation lines sug1-1 ZH11-GS2AA;proSUG1:SUG1. n = 56 in (g, h) n = 3 in i, n = 13 in (j, k). Data in (g–k) are presented as mean ± SD. Different letters represent significant differences (P < 0.05) from ordinary one-way ANOVA multiple comparisons with Tukey’s multiple comparisons test. Bars: 3 mm (a), 10 cm (b), 5 cm (c). Source data are provided as a Source data file.
