Fig. 1: URI is overexpressed in APC-loss driven human CRC, correlating with poor prognosis.
a URI1 mRNA levels in normal tissue (NT) and tumours from cancer patients, and fold change (FC) of expression in tumours compared to NT from TCGA samples. Stars denote the FDR-adjusted p. value. b URI1 mRNA in CRC tumours compared to matched normal tissue (n = 32). c Survival time in colorectal tumours expressing either high (n = 135) or low (n = 103) URI1 mRNA. Data are presented as mean ± SEM. d URI protein expression score from human CRC samples (n = 125), and its distribution according to tumour grade, Ki67 and AXIN2 scores, measured by IHC. e Association between Ki67 (left) and AXIN2 (right) expression scores with URI expression score. f Co-occurrences between high URI1 mRNA levels and APC mutations/loss, TP53 mutations/loss, KRAS mutations/gain in CRC samples from TCGA. g URI1 mRNA expression according to the consensus molecular subtype of CRC patients from TCGA. Odds ratio (OR) and p. value were calculated by fisher’s test. h Correlation between URI1 mRNA and patient survival in CMS2 patients. i Correlation between URI1 and MYC mRNA in CRC patients from TCGA. j Correlation between URI1 and CTNNB1 (upper) and AXIN2 (bottom) mRNA, in CRC patients from TCGA. k URI1 mRNA in human adenomas, serrated dysplasia and unclassified polyps (n = 28, 16, 11). l H&E and URI IHC of human adenomatous CRC polyps. m URI expression score in human colon pre-cancerous polyps and the adjacent non-polypoid mucosa (n = 75). n Detail of URI IHC of human adenomatous CRC polyp presenting non transformed and transformed cells with elongated nuclei. Dots represent individual patients. Statistical relevance of the correlations was assessed using Pearson correlation test. OR odds ratio, PCC Pearson correlation coefficient, p p. value. (***) = p. value < 0.005; (**) = p. value < 0.01. All statistical tests were two-sided. In linear correlations, grey shadow represents the 95% confidence interval of the linear regression fit. Boxplots represent Q1–Q3. Source data are provided as a Source Data file. Pictures of stainings are representative of the results obtained when staining and analysing all the samples available.
