Fig. 4: Structures in the IF^asym states.

a Overall view of the cryo-EM structure of MsRv1273c/72c in the AMPPNP-bound IF^asym−1 state. AMPPNP is shown as purple spheres. b Surface representation of the NBD dimer in the IF^asym−1 structure, viewed from the periplasm. The NBD of MsRv1273c is fixed as in Fig. 2g and the rotation (red arrow) of NBD of MsRv1272c is measured based on the position of the β-phosphorus atom of the nucleotide in the NBS (the angle between the two dashed lines linking β-phosphorus atoms at both sites). The degenerate site and consensus site are marked with red dashed circles. AMPPNP is shown as sticks. c Close-up view of the degenerate site in the IF^asym−1 structure. The Signature motif and Tyr343 of the A-loop are highlighted in blue and red, respectively. The shifts of α5 and α6 relative to the Occ structure in Fig. 3f are marked with red arrows. d Overall view of the cryo-EM structure of MsRv1273c/72c in the ADP-bound IF^asym-3 state. ADP is shown as green spheres. e Surface representation of NBD dimer in the IF^asym-3 structure. f Close-up view of the degenerate site in the IF^asym-3 structure. g Overall view of the cryo-EM structure of MsRv1273c/72c in the ATP | ADP-bound IF^asym-2 state. ATP and ADP are shown as yellow spheres. h Surface representation of NBD dimer in the IF^asym-2 structure. i Close-up view of the degenerate site in the IF^asym-2 structure.