Fig. 4: eSPR macrophages invigorate T cells. | Nature Communications

Fig. 4: eSPR macrophages invigorate T cells.

From: CAR macrophages with built-In CD47 blocker combat tumor antigen heterogeneity and activate T cells via cross-presentation

Fig. 4: eSPR macrophages invigorate T cells.

OVA/H2-Kb surface display on macrophages after phagocytosis. Representative scatter plots (a) and quantified ratio (b) of the surface OVA/H2-Kb expression (n = 3). c Macrophages surface OVA/H2-Kb display in correlation to phagocytosis. Pearson correlation was performed between the phagocytosis index and OVA/H2-Kb display ratios (n = 4). Proliferation of naïve OT-I (d) or OT-II (e) T cells cocultured with tumor-fed macrophages for 72 h (n = 3). f Schematic overview of MC38.HER2 carcinomatosis immunocompetent model. n = 15 for PBS, n = 10 for CD19.CAR, n = 11 for HER2.eSPR, n = 11 for B2M−/− HER2.eSPR. Data were pooled. g Kaplan–Meier survival curve of MC38.HER2 carcinomatosis with early treatment. h MC38 rechallenge for the survivors in the HER2.eSPR group or age-matched controls. i Schematic overview of MC38.HER2 carcinomatosis immunocompetent model. n = 5 for PBS, n = 5 for CD19.CAR, n = 13 for HER2.eSPR. j Kaplan–Meier survival curve of MC38.HER2 carcinomatosis with late treatment. k Schematic overview of MC38.HER2 carcinomatosis immunodeficient model. n = 4 for PBS, n = 5 for HER2.eSPR and B2M−/− HER2.eSPR. l Kaplan–Meier survival curve of MC38.HER2 carcinomatosis immunodeficient mice with early treatment. Representative scatter plots showing no apparent CD45.1 macrophage migration into lymph nodes (m) and phenotyping of dendritic cells (n) in tumor-draining lymph nodes (mediastinal-) and non-draining lymph nodes (inguinal-) from MC38.HER2 carcinomatosis-bearing mice with CD45.1 macrophage treatment (n = 4). o, p Flow cytometry phenotyping of lymphocytes from the tumor-draining lymph nodes (mediastinal-) and non-draining lymph nodes (inguinal-) from MC38.HER2 carcinomatosis-bearing mice with macrophage treatment (n = 4). Bars represent the mean and SD. Statistics were performed using one-way ANOVA with Tukey’s multiple comparisons for (n–p), two-way ANOVA with Tukey’s multiple comparisons for (b, d, and e); two-sided log-rank Mantel–Cox test without adjustment for (g, j, and l). Schematic illustrations were created in BioRender. Chen, S. (2025) https://BioRender.com/x52m488.

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