Fig. 5: Marchantia Mp-phy interacts with its PIF also through the Mp-phy^OPM-APA interaction.

a, b Y2H assay showing the interaction between Mp-phy and PIFs via the Mp-phy^OPM-APA interaction. BD-fused full-length Mp-phy, Mp-phy^PSM (1-610 a.a.), or Mp-phy^OPM (603-1126 a.a.) were transformed into AH109C with AD-fused Mp-PIF or MpPIF^mAPA (a), or with AD-fused PIF3, PIF3^mAPA, PIF3^mAPB, or PIF3^mAPA/mAPB (b). c, d Y2H assay showing the interaction between phyA and Mp-PIF (c) or phyB and Mp-PIF (d) via the phy^OPM-APA interaction. BD-fused full-length phyA, phyA^PSM, phy^OPM, phyB, phyB^PSM, or phyB^OPM were transformed into AH109C with AD-fused Mp-PIF or MpPIF^mAPA. Notations are consistent with those in Fig. 2a.